Broad Spectrum Inhibitor for Kinase Enrichment

Published by Luxi Zhang and co-workers in a 2013 paper (J.Proteome Research), their key reagent for stripping cell lysates by one-step affinity adsorption of kinases has been made available on a commercial basis as KiNet-1 (R). Here the active agent is immobilized to Sepharose beads and serves as a broad-band capture tool for kinases. The affinity tool may enrich more than 200 kinases in your cell lysates, depending on the cell line used. While the orginal method has been applied for analysis by mass spectrometry, analysis of abundant proteins by ELISA or Western blot is possible as well.

Customers in Europe may access KinEt-1 (R) through Artimmun Analytik GmbH, info [at artimmun.de]. Sets are available in pack sizes for 10-20-50 runs and include a detailed protocol.

Please refer to the following links for useful reference, including the original publication:

1) CTx0294885-Broad Spectrum Inhibitor as Affinity Reagent.  

2) Useful, updated standard procedure for in-gel digestion:

 


Signal Transduction

In Focus: ß-Catenin and AKT

 

ß-Catenin

ß-Catenin forms part of the cadherin / adhesion complex and presents a focal point in wnt signaling. It has been suggested that ß-catenin may also get involved with activation of the erk signaling pathway, supporting carcinogenesis. Dephospho ß-Catenin represents the active signaling part of the protein while post-translational accumulation has been placed under negative control through phosphorylation. This drives the protein towards ubiquitination and degradation.

Most of our signal transduction antibodies have been raised in, at least, two host animals and have been rigorously tested in Western Blot and Immunhistochemistry. A few are also suitable for immunoprecipitation.

Symansis ß catenin antibody from sheep shows a huge dynamic range in Western Blot. This has been exploited in a signaling array plate which offers flexible combination with antibodies for creb, GSK 3 alpha and beta or other parameters of interest: the Multi Kinase ELISA Array.  

 

Phospho AKT Pathway Sampler Kit, 950 Euro

 

E051002

5 x 50 ug

contains 5 individual antibodies
for IHC, WB raised against members of the AKT pathway   

pdf

 Get your quote for a pack of pathway samplers and save costs Inquire

AKT

AKT, also known as protein kinase B, takes a key role in Lipid signaling (specific: Phosphatidyl-3,4,5-trisphosphate PI345P3 and the bisphosphate PI34P2 as substrates), for example in neutrophils. A key element of AKT activation is phosphorylation at thr308. Involvement with other signaling pathways occurs in myocytes, natural killer cells and more. In particular, Symansis offers an antibody directed against a substrate that is a high molecular weight Rab GTPase activator. Antibody 3028 P2  detects this substrate which has been implied in the regulation of GLUT4 translocation. See below for the pdf and for a selection of AKT related antibodies

3028

1 x 100 ug

Anti-GTPase Activator

E051002

5 x 50 ug

Anti-Phospho-AKT sampler Kit

E011055

1 x 100 ug

anti-Phospho-AKT Thr308

 


Unphosphorylated ß-Catenin

Selective for Dephospho-ß-Catenin


Phospho-Apoptosis_Sampler






More Pathway Antibodies

 

 

 

 

 


Inhibitors, Methyltransferases, Kinases

 


Differentiation Targets-New Inhibitors & Inducers

  • Stem Cells
  • Epigenetic Targets
  • Autophagy
2014 inhibitor update-click image for reading


Kinase Pathway Quick Screen by Western ELISA

 

New Flexible Array Offers Combinatorial Signal Analysis

Assemble your array by selecting enough 2x8 well strips to set up a 96 well microtiter plate (see the menu on the picture below). Choose from 9 pre-assembled sets or focus on PI3 Kinase downstream signalling. A 2 hrs protocol yields semiquantitative data about selected phosphosignaling pathways in your cell lysate. Very economical, yet more sensitive and with a higher dynamic range than standard Western Blot read-outs, this colorimetric assay offers unique combinatorial set-ups, especially upon tandem use with Symansis inhibitors or Inhibitor Arrays.

 

new 96 well (6 x 16) Kinase Antibody ELISAs, each with specificity for up to 6 different phosphopeptides and active catenin

MKA 001

MKA002

MKA 003

MKA 004

MKA 005

Choose

AKT 1/2

GSK 3 a/ß

Dephospho Catenin

erk 1/2

 inhibitors available

MKA 006

MKA 007

MKA 008

MKA 010

CREB (pS133)

p38 alpha

GSK/beta Catenin

AKT / GSK


Kinase Pathway Screening Inhibitor Array

 

New Analyze the  response of your target cell against inhibitors

WIth its current focus on MAP Kinase, use Symansis inhibitor array to save time and costs. Optimize inhibitor use by maintaining a concentration window close to the EC50 of your target cell.

 

 


Research Tools Enzyme Inhibitors

A click on this link will produce an overview of Symansis Kinase Inhibitors available by us. You may also send a mail to info [at] artimmun.de for a comprehensive list covering other target proteins as well.

Choose from 300 + highly purified inhibitors, many addressed with their biological targets in a recent publication!

 

 


Kinases

 

 

Cat.No.

Description

Specificity

 [ug]

 €

RPK1001

AKT1, active 

Human

20

250

RPK1005

AKT1, unactive 

Human

20

180

RPK1010

AKT2, active

Human

100

250

RPK1015

AKT2, unactive 

Human

20

180

RPK1020

AKT3, active

Human

20

250

RPK1025

AMPKa, inactive 

Human

10

250

RPK1030

CHK2, active, E.coli

Human

20

250

RPK1035

CHK2, active, Sf9

Human

20

250

RPK1040

CK2 alpha 1, active

Human

10

150

RPK1045

CK2 alpha 2, active

Human

10

150

RPK1050

CK2 beta

Human

10

150

RPK1060

ERK1, active

Human

10

250

RPK1065

ERK1, inactive 

Human

20

150

RPK1070

ERK2, active

Human

5

250

RPK1075

ERK2, inactive

Human

10

150

RPK1080

JNK2alpha2, active

Human

100

250

RPK1085

MEK1, mutant, active

Human

10

250

RPK1090

MKK6, mutant, active

Human

20

250

RPK1095

p38alpha/SAPK2A, active

Human

20

250

RPK1100

p38alpha/SAPK2A,inactive

Human

20

150

RPK1110

PKA alpha, active catalytic

Human

50

250

 


 

 


Methyltransferases

 

 

 New Methyltransferase-Enzymes and Assays

 

Lys-Methyl
Transferase [G9A]

PRMT 1

PRDM2/RIZ1

Set 2

 

Top or Home

 

 

 

 

 



Symansis Inhibitors

Symansis PI3 Kinase Inhibitors

 

 

 

AUR (CDK)

 SY-JNJ7706621

JAK2

SY-TG101348

CDK

SY-AT-7519

FMS 

SY-Ki20227

MEK

SY-PD0325901

PI 3K beta

SY-TGX221

PI 3K p110alpha

SY-A66
Sun M et al (2010)PNAS 107,15547-52

 

Multikinase  [Motesanib]

SY-AMG706

 

 

 


More DNA Modifying Enzymes,

Nucleic Acid Extraction and Transfection